How to HRP Label and Purify Your Antibody - No HPLC Needed
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چکیده
Secondary conjugates are often employed to target a species specific antibody of interest (e.g., mouse, rabbit, goat, etc.). This method may save the researcher precious time in conjugation but secondary antibodies have their drawbacks. Secondary antibodies are known to significantly increase background signal through non-specific binding to antibody/antigen surfaces. For producing maximum specificity, with high signal to noise, it is always best to use direct primary antibody conjugates.
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تاریخ انتشار 2009